mouse fibroblasts  (ATCC)

Journal: Materials Today Bio

Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

doi: 10.1016/j.mtbio.2026.103182

Figure Lengend Snippet: Surface characterization of PPy/HA and PPy/cHA after hyaluronidase (HAase) treatment. (a) WCAs of gold, PPy/HA, and PPy/cHA before and after HAase treatment. (b) Quantification of surface carboxyl groups on PPy/HA and PPy/cHA after HAase treatment. (c) Electrochemical impedance spectra of gold, PPy/HA, and PPy/cHA electrodes with or without HAase treatment. (d) Relative impedance changes (%) at 1 Hz for PPy/HA and PPy/cHA electrodes after HAase treatment. The impedance value at 1 Hz of each electrode was normalized to that of HAase non-treated PPy/HA. (e) Representative optical micrographs of NIH-3T3 fibroblasts adhered on gold, PPy/HA, and PPy/cHA with and without HAase treatment. Scale bar = 200 μm. (f) Quantification of adhered cell numbers. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

Techniques:

Journal: Materials Today Bio

Article Title: Crosslinked hyaluronic acid-doped polypyrrole: Stable, nonbiofouling implantable bioelectrodes for in vivo signal recording

doi: 10.1016/j.mtbio.2026.103182

Figure Lengend Snippet: In vitro cytotoxicity tests. (a) Representative live/dead fluorescence images of NIH-3T3 fibroblasts cultured in extract media obtained from gold, PPy/HA, and PPy/cHA electrodes after 24 h of incubation. Live and dead cells are stained green and red, respectively. Scale bars = 200 μm. (b) Live cell percentages (cell viability). (c) Metabolic activity of cells assessed by WST-1 assay after 24 h incubation with extract media from gold, PPy/HA, and PPy/cHA electrodes. An asterisk (∗) denotes a statistically significant difference (p < 0.05). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Mouse NIH-3T3 fibroblasts (Korean Cell Line Bank, Seoul, Republic of Korea) were seeded on each sample to evaluate cell adhesion.

Techniques: In Vitro, Fluorescence, Cell Culture, Incubation, Staining, Activity Assay, WST-1 Assay

Journal: Science Advances

Article Title: International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity

doi: 10.1126/sciadv.aeb7039

Figure Lengend Snippet: ( A ) Schematic representation of the pipeline for growth factor candidate selection and cell-based testing. ( B ) Western blot analysis of 11 high-value CFPS-derived growth factors. FGF-1, FGF-2, FGF-10, TNF-α, IL-1β, IFN-γ, and IL-15 were expressed in E. coli BL21(DE3) in-house cell-free lysates, while IL-6, EGF, IGF-1, and IL-3 were expressed in SHuffle-based in-house cell-free lysates. Detection was performed using an anti–His–horseradish peroxidase (HRP) antibody (Ab). Representative data from one of three independent experiments. The molecular weight ladder (in kilodaltons) is shown on the left. ( C ) In vitro proliferation assay comparing on-demand, locally produced in Canada (blue) and commercial (green) FGF-1 growth factors in NIH-3T3 cells. Cells were treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml). Luminescence was measured using a conventional plate reader (means ± SD, n = 3). ( D ) In vitro proliferation assay comparing on-demand, locally produced (blue) and commercial (green) IL-3 growth factors in TF-1 cells. Cells were treated with varying concentrations (0.025, 0.050, 0.100, 0.5, 1, and 5 ng/ml). Representative data obtained using reagents produced on-site in Canada (means ± SD, n = 2). ( E ) Growth factor expression and cell-based validation in a low-resource setting. In vitro proliferation assay using FGF-1 and NIH-3T3 cells treated with varying concentrations (1, 5, 10, 50, 500, and 1000 ng/ml) of in-house–produced (blue) and commercial (green) growth factors (means ± SD, n = 3). Representative data obtained using reagents produced on-site in Brazil. Relative fold proliferation was plotted relative to untreated, serum-starved cells under the same experimental conditions. Two-way ANOVA determined statistical differences with Šídák’s post hoc multiple comparisons test (ns, P > 0.05; * P < 0.05; *** P < 0.001).

Article Snippet: Vero CCL-81 [American Type Culture Collection (ATCC), CCL-81] and NIH-3T3 mouse fibroblast (ATCC, CRL-1658) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco).

Techniques: Selection, Western Blot, Derivative Assay, Molecular Weight, In Vitro, Proliferation Assay, Produced, Expressing, Biomarker Discovery